horseradish peroxidase reaction with hydrogen peroxide

2 This property of heme peroxidases make them useful for attaching to “transparent” proteins so that they can be seen under different wavelengths.

KEILIN D, HARTREE EF.

The mechanism of the reaction of horseradish peroxidase isoenzyme C (HRPC) with hydrogen peroxide to form the reactive enzyme intermediate compound I has been studied using electronic absorbance, rapid-scan stopped-flow, and electron paramagnetic resonance (EPR) spectroscopies at both acid and basic pH. Purification of horse-radish peroxidase and comparison of its properties with those of catalase and methaemoglobin.

Horseradish peroxidase uses hydrogen peroxide to oxidize both organic and inorganic compounds. Adediran SA, Lambeir AM.

1 Horseradish peroxidase along with other heme peroxidases are brightly colored especially under the near-ultraviolet light. To observe the formation process of compound I in horseradish peroxidase (HRP), we developed a new freeze-quench device with ∼200 μs of the mixing-to-freezing time interval and observed the reaction between HRP and hydrogen peroxide (H 2 O 2).The developed device consists of a submillisecond solution mixer and rotating copper or silver plates cooled at 77 K; it freezes the small … Kinetics of the reaction of compound II of horseradish peroxidase with hydrogen peroxide to form compound III.

Peroxidases are found in all plants and animals, and are essential for living systems. Eur J Biochem. HRP (donor:hydrogen peroxide oxidoreductase; EC 1.11.1.7), derived from horseradish roots, is a enzyme of molecular weight 40,000 that can catalyze the reaction of hydrogen peroxide with certain organic, electron-donating substrates to yield highly colored products (Figure 22.1).

Peroxidase activity was discovered by Schonbein in 1855 when treating guaiacol with hydrogen peroxide and plant extracts. 1989 Dec 22; 186 (3):571–576. Introduction.

HRP readily combines with hydrogen peroxide (H 2 O 2), and the resultant [HRP–H 2 O 2] complex can oxidize a wide variety of chromogenic hydrogen donors. It can also utilize chemiluminescent substrates such as luminol and isoluminol, and fluorogenic substrates such as tyramine, homovanillic acid, and 4–hydroxyphenyl acetic acid.


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